Publications

2026

Nsamba ET, Villeneuve AM. Differential contributions of β-tubulin isotypes to acentrosomal oocyte meiosis in C. elegans. J Cell Biol. 2026 Oct 5;225(10):e202511199, https://doi.org/10.1083/jcb.202511199

Gonzalez de la Rosa P, Strand LG, Stevens L, Kieninger M, Collins J, Johansen M, Chan TW, Adams S, Villeneuve AM, Pires da Silva A, Blaxter M. Massive programmed DNA elimination during embryogenesis in the trioecious nematode Auanema rhodense. bioRxiv. 2026 Aug 28, https://doi.org/10.64898/2026.08.27.747507

Strand LG, Choi CP, McCoy S, Nsamba ET, Silva N, & Villeneuve AM. Active maintenance of meiosis-specific chromosome structures in Caenorhabditis elegans by the deubiquitinase DUO-1, Proc. Natl. Acad. Sci. U.S.A. 2026 Mar 24;123 (12) e2532671123, https://doi.org/10.1073/pnas.2532671123

2023

Yamaya K, Wang B, Memar N, Odiba AS, Woglar A, Gartner A, Villeneuve AM. Disparate roles for C. elegans DNA translocase paralogs RAD-54.L and RAD-54.B in meiotic prophase germ cells. Nucleic Acids Res. 2023 Sep 22;51(17):9183-9202. doi: 10.1093/nar/gkad638. PMID: 37548405; PMCID: PMC10516670. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10516670/

Cahoon, C. K., Uebel, C. J., Villeneuve, A.M., Libuda, D. E. Epitope tag-specific differences in the detection of COSA-1 marked crossover sites in C. elegans spermatocytes. microPublication Biology (2023). https://pubmed.ncbi.nlm.nih.gov/36660421

Choi, C. P., Villeneuve, A.M. CRISPR/Cas9 mediated genome editing of Caenorhabditis nigoni using the conserved dpy-10 co-conversion marker. microPublication Biology (2023). https://pubmed.ncbi.nlm.nih.gov/37720684/

2022

Roelens, B. & Villeneuve, A. M. Localization of HIM-19 in the C. elegans germline. MicroPubl Biol 2022 (2022). https://www.ncbi.nlm.nih.gov/pubmed/36035776

Haversat, J., Woglar, A., Klatt, K., Akerib, C. C., Roberts, V., Chen, S. Y., Arur, S., Villeneuve, A. M. & Kim, Y. Robust designation of meiotic crossover sites by CDK-2 through phosphorylation of the MutSgamma complex. Proc Natl Acad Sci U S A 119, e2117865119 (2022). https://www.ncbi.nlm.nih.gov/pubmed/35576467

Akerib C.C., Yokoo R., Nsamba E.T., Strand L.G., Yamaya K., Villeneuve A.M. A new partial loss of function allele of rad-54.L. microPublication Biology (2022). https://pubmed.ncbi.nlm.nih.gov/36247323/

2021

Girard, C., Akerib, C. C. & Villeneuve, A. M. Suppression of him-14(it44ts) by a transgene insertion expressing GFP::COSA-1. MicroPubl Biol 2021 (2021). https://www.ncbi.nlm.nih.gov/pubmed/34458691

Hinman, A. W., Yeh, H. Y., Roelens, B., Yamaya, K., Woglar, A., Bourbon, H. G., Chi, P. & Villeneuve, A. M. Caenorhabditis elegans DSB-3 reveals conservation and divergence among protein complexes promoting meiotic double-strand breaks. Proc Natl Acad Sci U S A 118 (2021). https://www.ncbi.nlm.nih.gov/pubmed/34389685

2020

Woglar, A., Yamaya, K., Roelens, B., Boettiger, A., Kohler, S. & Villeneuve, A. M. Quantitative cytogenetics reveals molecular stoichiometry and longitudinal organization of meiotic chromosome axes and loops. PLoS Biol 18, e3000817 (2020). https://www.ncbi.nlm.nih.gov/pubmed/32813728

2019

Roelens, B., Barroso, C., Montoya, A., Cutillas, P., Zhang, W., Woglar, A., Girard, C., Martinez-Perez, E. & Villeneuve, A. M. Spatial Regulation of Polo-Like Kinase Activity During Caenorhabditis elegans Meiosis by the Nucleoplasmic HAL-2/HAL-3 Complex. Genetics 213, 79-96 (2019). https://www.ncbi.nlm.nih.gov/pubmed/31345995

Roelens, B., Zawadzki, K. & Villeneuve, A. me98 is a new allele of rad-54. MicroPubl Biol 2019 (2019). https://www.ncbi.nlm.nih.gov/pubmed/32550460

Roelens, B., Zawadzki, K. & Villeneuve, A. me101 is a new allele of rad-51. MicroPubl Biol 2019 (2019). https://www.ncbi.nlm.nih.gov/pubmed/32550442

2018

Girard, C., Roelens, B., Zawadzki, K. A. & Villeneuve, A. M. Interdependent and separable functions of Caenorhabditis elegans MRN-C complex members couple formation and repair of meiotic DSBs. Proc Natl Acad Sci U S A 115, E4443-E4452 (2018). https://www.ncbi.nlm.nih.gov/pubmed/29686104

Woglar, A. & Villeneuve, A. M. Dynamic Architecture of DNA Repair Complexes and the Synaptonemal Complex at Sites of Meiotic Recombination. Cell 173, 1678-1691 e1616 (2018). https://www.ncbi.nlm.nih.gov/pubmed/29754818

2017

Mlynarczyk-Evans, S. & Villeneuve, A. M. Time-Course Analysis of Early Meiotic Prophase Events Informs Mechanisms of Homolog Pairing and Synapsis in Caenorhabditis elegans. Genetics 207, 103-114 (2017). https://www.ncbi.nlm.nih.gov/pubmed/28710064

Pattabiraman, D., Roelens, B., Woglar, A. & Villeneuve, A. M. Meiotic recombination modulates the structure and dynamics of the synaptonemal complex during C. elegans meiosis. PLoS Genet 13, e1006670 (2017). https://www.ncbi.nlm.nih.gov/pubmed/28339470

2016

Gabdank, I., Ramakrishnan, S., Villeneuve, A. M. & Fire, A. Z. A streamlined tethered chromosome conformation capture protocol. BMC Genomics 17, 274 (2016). https://www.ncbi.nlm.nih.gov/pubmed/27036078

Jagut, M., Hamminger, P., Woglar, A., Millonigg, S., Paulin, L., Mikl, M., Dello Stritto, M. R., Tang, L., Habacher, C., Tam, A., Gallach, M., von Haeseler, A., Villeneuve, A. M. & Jantsch, V. Separable Roles for a Caenorhabditis elegans RMI1 Homolog in Promoting and Antagonizing Meiotic Crossovers Ensure Faithful Chromosome Inheritance. PLoS Biol 14, e1002412 (2016). https://www.ncbi.nlm.nih.gov/pubmed/27011106

Wolff, I. D., Tran, M. V., Mullen, T. J., Villeneuve, A. M. & Wignall, S. M. Assembly of Caenorhabditis elegans acentrosomal spindles occurs without evident microtubule-organizing centers and requires microtubule sorting by KLP-18/kinesin-12 and MESP-1. Mol Biol Cell 27, 3122-3131 (2016). https://www.ncbi.nlm.nih.gov/pubmed/27559133

2015

Roelens, B., Schvarzstein, M. & Villeneuve, A. M. Manipulation of Karyotype in Caenorhabditis elegans Reveals Multiple Inputs Driving Pairwise Chromosome Synapsis During Meiosis. Genetics 201, 1363-1379 (2015). https://www.ncbi.nlm.nih.gov/pubmed/26500263

2014

Holloway, J. K., Sun, X., Yokoo, R., Villeneuve, A. M. & Cohen, P. E. Mammalian CNTD1 is critical for meiotic crossover maturation and deselection of excess precrossover sites. J Cell Biol 205, 633-641 (2014). https://www.ncbi.nlm.nih.gov/pubmed/24891606

Schvarzstein, M., Pattabiraman, D., Libuda, D. E., Ramadugu, A., Tam, A., Martinez-Perez, E., Roelens, B., Zawadzki, K. A., Yokoo, R., Rosu, S., Severson, A. F., Meyer, B. J., Nabeshima, K. & Villeneuve, A. M. DNA helicase HIM-6/BLM both promotes MutSgamma-dependent crossovers and antagonizes MutSgamma-independent interhomolog associations during Caenorhabditis elegans meiosis. Genetics 198, 193-207 (2014). https://www.ncbi.nlm.nih.gov/pubmed/25053665

2013

Bilgir, C., Dombecki, C. R., Chen, P. F., Villeneuve, A. M. & Nabeshima, K. Assembly of the Synaptonemal Complex Is a Highly Temperature-Sensitive Process That Is Supported by PGL-1 During Caenorhabditis elegans Meiosis. G3 (Bethesda) 3, 585-595 (2013). https://www.ncbi.nlm.nih.gov/pubmed/23550120

Labrador, L., Barroso, C., Lightfoot, J., Muller-Reichert, T., Flibotte, S., Taylor, J., Moerman, D. G., Villeneuve, A. M. & Martinez-Perez, E. Chromosome movements promoted by the mitochondrial protein SPD-3 are required for homology search during Caenorhabditis elegans meiosis. PLoS Genet 9, e1003497 (2013). https://www.ncbi.nlm.nih.gov/pubmed/23671424

Libuda, D. E., Uzawa, S., Meyer, B. J. & Villeneuve, A. M. Meiotic chromosome structures constrain and respond to designation of crossover sites. Nature 502, 703-706 (2013). https://www.ncbi.nlm.nih.gov/pubmed/24107990

Mlynarczyk-Evans, S., Roelens, B. & Villeneuve, A. M. Evidence that masking of synapsis imperfections counterbalances quality control to promote efficient meiosis. PLoS Genet 9, e1003963 (2013). https://www.ncbi.nlm.nih.gov/pubmed/24339786

Rosu, S., Zawadzki, K. A., Stamper, E. L., Libuda, D. E., Reese, A. L., Dernburg, A. F. & Villeneuve, A. M. The C. elegans DSB-2 protein reveals a regulatory network that controls competence for meiotic DSB formation and promotes crossover assurance. PLoS Genet 9, e1003674 (2013). https://www.ncbi.nlm.nih.gov/pubmed/23950729

Schvarzstein, M., Pattabiraman, D., Bembenek, J. N. & Villeneuve, A. M. Meiotic HORMA domain proteins prevent untimely centriole disengagement during Caenorhabditis elegans spermatocyte meiosis. Proc Natl Acad Sci U S A 110, E898-907 (2013). https://www.ncbi.nlm.nih.gov/pubmed/23401519

Stamper, E. L., Rodenbusch, S. E., Rosu, S., Ahringer, J., Villeneuve, A. M. & Dernburg, A. F. Identification of DSB-1, a protein required for initiation of meiotic recombination in Caenorhabditis elegans, illuminates a crossover assurance checkpoint. PLoS Genet 9, e1003679 (2013). https://www.ncbi.nlm.nih.gov/pubmed/23990794

2012

Voelkel-Meiman, K., Moustafa, S. S., Lefrancois, P., Villeneuve, A. M. & MacQueen, A. J. Full-length synaptonemal complex grows continuously during meiotic prophase in budding yeast. PLoS Genet 8, e1002993 (2012). https://www.ncbi.nlm.nih.gov/pubmed/23071451

Yokoo, R., Zawadzki, K. A., Nabeshima, K., Drake, M., Arur, S. & Villeneuve, A. M. COSA-1 reveals robust homeostasis and separable licensing and reinforcement steps governing meiotic crossovers. Cell 149, 75-87 (2012). https://www.ncbi.nlm.nih.gov/pubmed/22464324

Zhang, W., Miley, N., Zastrow, M. S., MacQueen, A. J., Sato, A., Nabeshima, K., Martinez-Perez, E., Mlynarczyk-Evans, S., Carlton, P. M. & Villeneuve, A. M. HAL-2 promotes homologous pairing during Caenorhabditis elegans meiosis by antagonizing inhibitory effects of synaptonemal complex precursors. PLoS Genet 8, e1002880 (2012). https://www.ncbi.nlm.nih.gov/pubmed/22912597

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