Publications
2023
Yamaya K, Wang B, Memar N, Odiba AS, Woglar A, Gartner A, Villeneuve AM. Disparate roles for C. elegans DNA translocase paralogs RAD-54.L and RAD-54.B in meiotic prophase germ cells. Nucleic Acids Res. 2023 Sep 22;51(17):9183-9202. doi: 10.1093/nar/gkad638. PMID: 37548405; PMCID: PMC10516670. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10516670/
Cahoon, C. K., Uebel, C. J., Villeneuve, A.M., Libuda, D. E. Epitope tag-specific differences in the detection of COSA-1 marked crossover sites in C. elegans spermatocytes. microPublication Biology (2023). https://pubmed.ncbi.nlm.nih.gov/36660421
Choi, C. P., Villeneuve, A.M. CRISPR/Cas9 mediated genome editing of Caenorhabditis nigoni using the conserved dpy-10 co-conversion marker. microPublication Biology (2023). https://pubmed.ncbi.nlm.nih.gov/37720684/
2022
Roelens, B. & Villeneuve, A. M. Localization of HIM-19 in the C. elegans germline. MicroPubl Biol 2022 (2022). https://www.ncbi.nlm.nih.gov/pubmed/36035776
Haversat, J., Woglar, A., Klatt, K., Akerib, C. C., Roberts, V., Chen, S. Y., Arur, S., Villeneuve, A. M. & Kim, Y. Robust designation of meiotic crossover sites by CDK-2 through phosphorylation of the MutSgamma complex. Proc Natl Acad Sci U S A 119, e2117865119 (2022). https://www.ncbi.nlm.nih.gov/pubmed/35576467
Akerib C.C., Yokoo R., Nsamba E.T., Strand L.G., Yamaya K., Villeneuve A.M. A new partial loss of function allele of rad-54.L. microPublication Biology (2022). https://pubmed.ncbi.nlm.nih.gov/36247323/
2021
Girard, C., Akerib, C. C. & Villeneuve, A. M. Suppression of him-14(it44ts) by a transgene insertion expressing GFP::COSA-1. MicroPubl Biol 2021 (2021). https://www.ncbi.nlm.nih.gov/pubmed/34458691
Hinman, A. W., Yeh, H. Y., Roelens, B., Yamaya, K., Woglar, A., Bourbon, H. G., Chi, P. & Villeneuve, A. M. Caenorhabditis elegans DSB-3 reveals conservation and divergence among protein complexes promoting meiotic double-strand breaks. Proc Natl Acad Sci U S A 118 (2021). https://www.ncbi.nlm.nih.gov/pubmed/34389685
2020
Woglar, A., Yamaya, K., Roelens, B., Boettiger, A., Kohler, S. & Villeneuve, A. M. Quantitative cytogenetics reveals molecular stoichiometry and longitudinal organization of meiotic chromosome axes and loops. PLoS Biol 18, e3000817 (2020). https://www.ncbi.nlm.nih.gov/pubmed/32813728
2019
Roelens, B., Barroso, C., Montoya, A., Cutillas, P., Zhang, W., Woglar, A., Girard, C., Martinez-Perez, E. & Villeneuve, A. M. Spatial Regulation of Polo-Like Kinase Activity During Caenorhabditis elegans Meiosis by the Nucleoplasmic HAL-2/HAL-3 Complex. Genetics 213, 79-96 (2019). https://www.ncbi.nlm.nih.gov/pubmed/31345995
Roelens, B., Zawadzki, K. & Villeneuve, A. me98 is a new allele of rad-54. MicroPubl Biol 2019 (2019). https://www.ncbi.nlm.nih.gov/pubmed/32550460
Roelens, B., Zawadzki, K. & Villeneuve, A. me101 is a new allele of rad-51. MicroPubl Biol 2019 (2019). https://www.ncbi.nlm.nih.gov/pubmed/32550442
2018
Girard, C., Roelens, B., Zawadzki, K. A. & Villeneuve, A. M. Interdependent and separable functions of Caenorhabditis elegans MRN-C complex members couple formation and repair of meiotic DSBs. Proc Natl Acad Sci U S A 115, E4443-E4452 (2018). https://www.ncbi.nlm.nih.gov/pubmed/29686104
Woglar, A. & Villeneuve, A. M. Dynamic Architecture of DNA Repair Complexes and the Synaptonemal Complex at Sites of Meiotic Recombination. Cell 173, 1678-1691 e1616 (2018). https://www.ncbi.nlm.nih.gov/pubmed/29754818
2017
Mlynarczyk-Evans, S. & Villeneuve, A. M. Time-Course Analysis of Early Meiotic Prophase Events Informs Mechanisms of Homolog Pairing and Synapsis in Caenorhabditis elegans. Genetics 207, 103-114 (2017). https://www.ncbi.nlm.nih.gov/pubmed/28710064
Pattabiraman, D., Roelens, B., Woglar, A. & Villeneuve, A. M. Meiotic recombination modulates the structure and dynamics of the synaptonemal complex during C. elegans meiosis. PLoS Genet 13, e1006670 (2017). https://www.ncbi.nlm.nih.gov/pubmed/28339470
2016
Gabdank, I., Ramakrishnan, S., Villeneuve, A. M. & Fire, A. Z. A streamlined tethered chromosome conformation capture protocol. BMC Genomics 17, 274 (2016). https://www.ncbi.nlm.nih.gov/pubmed/27036078
Jagut, M., Hamminger, P., Woglar, A., Millonigg, S., Paulin, L., Mikl, M., Dello Stritto, M. R., Tang, L., Habacher, C., Tam, A., Gallach, M., von Haeseler, A., Villeneuve, A. M. & Jantsch, V. Separable Roles for a Caenorhabditis elegans RMI1 Homolog in Promoting and Antagonizing Meiotic Crossovers Ensure Faithful Chromosome Inheritance. PLoS Biol 14, e1002412 (2016). https://www.ncbi.nlm.nih.gov/pubmed/27011106
Wolff, I. D., Tran, M. V., Mullen, T. J., Villeneuve, A. M. & Wignall, S. M. Assembly of Caenorhabditis elegans acentrosomal spindles occurs without evident microtubule-organizing centers and requires microtubule sorting by KLP-18/kinesin-12 and MESP-1. Mol Biol Cell 27, 3122-3131 (2016). https://www.ncbi.nlm.nih.gov/pubmed/27559133
2015
Roelens, B., Schvarzstein, M. & Villeneuve, A. M. Manipulation of Karyotype in Caenorhabditis elegans Reveals Multiple Inputs Driving Pairwise Chromosome Synapsis During Meiosis. Genetics 201, 1363-1379 (2015). https://www.ncbi.nlm.nih.gov/pubmed/26500263
2014
Holloway, J. K., Sun, X., Yokoo, R., Villeneuve, A. M. & Cohen, P. E. Mammalian CNTD1 is critical for meiotic crossover maturation and deselection of excess precrossover sites. J Cell Biol 205, 633-641 (2014). https://www.ncbi.nlm.nih.gov/pubmed/24891606
Schvarzstein, M., Pattabiraman, D., Libuda, D. E., Ramadugu, A., Tam, A., Martinez-Perez, E., Roelens, B., Zawadzki, K. A., Yokoo, R., Rosu, S., Severson, A. F., Meyer, B. J., Nabeshima, K. & Villeneuve, A. M. DNA helicase HIM-6/BLM both promotes MutSgamma-dependent crossovers and antagonizes MutSgamma-independent interhomolog associations during Caenorhabditis elegans meiosis. Genetics 198, 193-207 (2014). https://www.ncbi.nlm.nih.gov/pubmed/25053665
2013
Bilgir, C., Dombecki, C. R., Chen, P. F., Villeneuve, A. M. & Nabeshima, K. Assembly of the Synaptonemal Complex Is a Highly Temperature-Sensitive Process That Is Supported by PGL-1 During Caenorhabditis elegans Meiosis. G3 (Bethesda) 3, 585-595 (2013). https://www.ncbi.nlm.nih.gov/pubmed/23550120
Labrador, L., Barroso, C., Lightfoot, J., Muller-Reichert, T., Flibotte, S., Taylor, J., Moerman, D. G., Villeneuve, A. M. & Martinez-Perez, E. Chromosome movements promoted by the mitochondrial protein SPD-3 are required for homology search during Caenorhabditis elegans meiosis. PLoS Genet 9, e1003497 (2013). https://www.ncbi.nlm.nih.gov/pubmed/23671424
Libuda, D. E., Uzawa, S., Meyer, B. J. & Villeneuve, A. M. Meiotic chromosome structures constrain and respond to designation of crossover sites. Nature 502, 703-706 (2013). https://www.ncbi.nlm.nih.gov/pubmed/24107990
Mlynarczyk-Evans, S., Roelens, B. & Villeneuve, A. M. Evidence that masking of synapsis imperfections counterbalances quality control to promote efficient meiosis. PLoS Genet 9, e1003963 (2013). https://www.ncbi.nlm.nih.gov/pubmed/24339786
Rosu, S., Zawadzki, K. A., Stamper, E. L., Libuda, D. E., Reese, A. L., Dernburg, A. F. & Villeneuve, A. M. The C. elegans DSB-2 protein reveals a regulatory network that controls competence for meiotic DSB formation and promotes crossover assurance. PLoS Genet 9, e1003674 (2013). https://www.ncbi.nlm.nih.gov/pubmed/23950729
Schvarzstein, M., Pattabiraman, D., Bembenek, J. N. & Villeneuve, A. M. Meiotic HORMA domain proteins prevent untimely centriole disengagement during Caenorhabditis elegans spermatocyte meiosis. Proc Natl Acad Sci U S A 110, E898-907 (2013). https://www.ncbi.nlm.nih.gov/pubmed/23401519
Stamper, E. L., Rodenbusch, S. E., Rosu, S., Ahringer, J., Villeneuve, A. M. & Dernburg, A. F. Identification of DSB-1, a protein required for initiation of meiotic recombination in Caenorhabditis elegans, illuminates a crossover assurance checkpoint. PLoS Genet 9, e1003679 (2013). https://www.ncbi.nlm.nih.gov/pubmed/23990794
2012
Voelkel-Meiman, K., Moustafa, S. S., Lefrancois, P., Villeneuve, A. M. & MacQueen, A. J. Full-length synaptonemal complex grows continuously during meiotic prophase in budding yeast. PLoS Genet 8, e1002993 (2012). https://www.ncbi.nlm.nih.gov/pubmed/23071451
Yokoo, R., Zawadzki, K. A., Nabeshima, K., Drake, M., Arur, S. & Villeneuve, A. M. COSA-1 reveals robust homeostasis and separable licensing and reinforcement steps governing meiotic crossovers. Cell 149, 75-87 (2012). https://www.ncbi.nlm.nih.gov/pubmed/22464324
Zhang, W., Miley, N., Zastrow, M. S., MacQueen, A. J., Sato, A., Nabeshima, K., Martinez-Perez, E., Mlynarczyk-Evans, S., Carlton, P. M. & Villeneuve, A. M. HAL-2 promotes homologous pairing during Caenorhabditis elegans meiosis by antagonizing inhibitory effects of synaptonemal complex precursors. PLoS Genet 8, e1002880 (2012). https://www.ncbi.nlm.nih.gov/pubmed/22912597